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KMID : 0363220130510100776
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Korean Journal of Dermatology
2013 Volume.51 No. 10 p.776 ~ p.781
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Immunohistochemical Study for the Syndecan-2 in Cutaneous Melanoma and Pigmented Nevus
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Yun Woo-Jin
Choi So-Ra
Noh Tai-Kyung
Park Ho-Young
Choi Jung-Ran
Chang Sung-Eun
Lee Mi-Woo
Choi Jee-Ho
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Abstract
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Background: Melanoma is a malignant neoplasm originating from melanocytes. It has been recently suggested that syndecan-2 may contribute to the aggressive phenotype and metastatic potential of melanoma in cell line studies. However, there is no quantitative analysis of syndecan-2 expression using human melanoma tissue.
Objective: This study aimed to examine the specific expression of syndecan-2 in human melanoma tissue.
Methods: A total of 35 sections of formalin-fixed, paraffin-embedded tissues were investigated for syndecan-2 expression using immunohistochemical staining. Also, a total of 6 tissues and two kinds of cell lines were analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR) and western blot.
Results: Immunohistochemical staining of 23 cases of melanoma tissue was done, and in 5 cases (21.7%), strong expression of syndecan-2 was seen. Also, syndecan-2 was detected in human melanoma tissue and MNT-1 melanoma cells by RT-PCR and western blot analysis.
Conclusion: We suggest that syndecan-2 expression is increased in melanoma compared to nevus. The results of this study may help to explain the clinical features of melanoma and syndecan-2.
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KEYWORD
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Melanoma, Syndecan-2
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